Weber Medicine & Clinical Case Reports

April 2015, Vol. 2 (1), ISSN:2449-1624

© Author(s) 2016. This work is distributed under the Creative Commons Attribution 3.0 License.

Research Article

Rapid enzimatic disruption of Enterococcus faecalis biofilm using Carica pubescens: a pilot study

Karla Elena Torres-Chávez 2,3 & Gustavo Alberto Obando-Pereda 1,2

1 Dentistry School of Saint Mary Catholic University.

2 Dentistry School of Andean University of Cusco.

3 Dentistry School of Universidad Alas Peruanas.

Accepted 16th April, 2016; Available Online 19th April, 2016.


In endodontic practice, the continue endodontic failure can be produce by the presence of Enterococcus faecalis in the root complex. The aim of this study was to kill E. faecalis in biofilm state using a proteolytic agent. A biofilm model was conducted for E. faecalis growing and then was employed endondontic irrigants as clorhexidine 2%, sodium hypoclorite 5% and C. pubescens 2% for disrupt the E. faecalis biofilm at 2, 5 and 10 minutes. Turbidity method and colony formed unit where employed to measure the disruption. C. pubescens 2% showed the most disruption of E. faecalis biofilm at 2, 5 and 10 minutes. Sodium hypoclorite 5% showed bacterial disruption at 10 minutes. Clorhexidine 2% not showed biofilm disruption at all times. Conclusion: enzymatic treatment for total disruption of E. faecalis may be the ideal therapy for infected root canals.

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